pg-ODD-EGFP
(Plasmid #87211)

• Purpose: HIF-1a's ODD domain fused to EGFP
• Depositing Lab: Violaine Sée
• Publication: Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64. doi: 10.1074/jbc.M113.500405. Epub 2014 Jan 6.

Backbone

• Vector backbone: unknown
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIF-1a ODD domain
• Species: H. sapiens (human)
• Mutation: ODD domain (AA 530-652)
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry