pg-proPHD2-PHD2-EGFP
(Plasmid #87210)

• Purpose: PHD2-EGFP expressed under the control of the PHD2 promoter
• Depositing Lab: Violaine Sée
• Publication: Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64. doi: 10.1074/jbc.M113.500405. Epub 2014 Jan 6.

Backbone

• Vector backbone: unknown
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHD2
• Species: H. sapiens (human)
• Mutation: H313N
• Entrez Gene: EGLN1 (a.k.a. C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6)
• Promoter: PHD2
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: EGFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PHD2-EGFP was a gift from Dr. Reinhard Depping, Universitaet zu Luebeck, Germany