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Addgene

pg-dsRedXP-HIF-2alpha
(Plasmid #87209)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87209 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIF-2a
  • Species
    H. sapiens (human)
  • Mutation
    Q868E
  • Entrez Gene
    EPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
  • Promoter CMV
  • Tag / Fusion Protein
    • dsRedXP (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HIF2a donated by Dr. S. McKnight, UT Southwestern Medical Center

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pg-dsRedXP-HIF-2alpha was a gift from Violaine Sée (Addgene plasmid # 87209 ; http://n2t.net/addgene:87209 ; RRID:Addgene_87209)
  • For your References section:

    Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Taylor SE, Bagnall J, Mason D, Levy R, Fernig DG, See V. Open Biol. 2016 Sep;6(9). pii: 160195. 10.1098/rsob.160195 PubMed 27655733