pg-dsRedXP-HIF-2alpha
(Plasmid
#87209)
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PurposeN-terminally dsRedXP-tagged human HIF-2a/EPAS
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIF-2a
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SpeciesH. sapiens (human)
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MutationQ868E
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Entrez GeneEPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
- Promoter CMV
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Tag
/ Fusion Protein
- dsRedXP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HIF2a donated by Dr. S. McKnight, UT Southwestern Medical Center
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pg-dsRedXP-HIF-2alpha was a gift from Violaine Sée (Addgene plasmid # 87209 ; http://n2t.net/addgene:87209 ; RRID:Addgene_87209) -
For your References section:
Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Taylor SE, Bagnall J, Mason D, Levy R, Fernig DG, See V. Open Biol. 2016 Sep;6(9). pii: 160195. 10.1098/rsob.160195 PubMed 27655733