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Addgene

pg-HIF-1alpha-EGFP
(Plasmid #87204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIF-1a
  • Species
    H. sapiens (human)
  • Mutation
    L40F, M88V, Q814R
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pg-HIF-1alpha-EGFP was a gift from Violaine Sée (Addgene plasmid # 87204 ; http://n2t.net/addgene:87204 ; RRID:Addgene_87204)

  • For your References section:

    Tight control of hypoxia-inducible factor-alpha transient dynamics is essential for cell survival in hypoxia. Bagnall J, Leedale J, Taylor SE, Spiller DG, White MR, Sharkey KJ, Bearon RN, See V. J Biol Chem. 2014 Feb 28;289(9):5549-64. doi: 10.1074/jbc.M113.500405. Epub 2014 Jan 6. 10.1074/jbc.M113.500405 PubMed 24394419
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