pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)
(Plasmid #87203)

• Purpose: To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pEGFP-C1
• Modifications to backbone: A FLAG tag is added on N-terminus of EGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBL4A
• Alt name: Ubiquitin-like protein 4A
• Alt name: Ubiquitin-like protein GDX
• Species: H. sapiens (human)
• Insert Size (bp): 69
• Mutation: Residues from 108-130 are inserted.
• GenBank ID: NM_014235.4
• Entrez Gene: UBL4A (a.k.a. DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-pArev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry