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Addgene

pCDNA-H1-sgRNA-mTZAP
(Plasmid #87187)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87187 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA-H1-sgRNA
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TZAP
  • gRNA/shRNA sequence
    TGTGGGACATTCGACCGGAA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Zbtb48 (a.k.a. 0610011D15Rik, AI327031, Hkr3, TZAP)
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer H1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-H1-sgRNA-mTZAP was a gift from Eros Lazzerini Denchi (Addgene plasmid # 87187 ; http://n2t.net/addgene:87187 ; RRID:Addgene_87187)

  • For your References section:

    TZAP: A telomere-associated protein involved in telomere length control. Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder J, Lazzerini Denchi E. Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752. 10.1126/science.aah6752 PubMed 28082411
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