sv272-HisMBP-TZAPZNF9-11
(Plasmid
#87185)
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Purposebacterial expression of HisMBP-TZAPZNF9-11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonesv272
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTZAP
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SpeciesH. sapiens (human)
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Entrez GeneZBTB48 (a.k.a. HKR3, TZAP, ZNF855, pp9964)
- Promoter T7
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Tag
/ Fusion Protein
- HisMBP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer MBP-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sv272-HisMBP-TZAPZNF9-11 was a gift from Eros Lazzerini Denchi (Addgene plasmid # 87185 ; http://n2t.net/addgene:87185 ; RRID:Addgene_87185) -
For your References section:
TZAP: A telomere-associated protein involved in telomere length control. Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder J, Lazzerini Denchi E. Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752. 10.1126/science.aah6752 PubMed 28082411