pSMART-ala12
(Plasmid
#87173)
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PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMART-HCKan (AF532107.1)
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Backbone manufacturerLucigen (Middleton, WI)
- Backbone size w/o insert (bp) 1800
- Total vector size (bp) 6256
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNADPH Alanine Dehydrogenase
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Alt nameald*
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SpeciesSynthetic
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Insert Size (bp)1200
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MutationD196A, L197R
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Entrez Geneald (a.k.a. BSU31930)
- Promoter Constitutive proC
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CAG TCC AGT TAC GCT GGA GTC
- 3′ sequencing primer GGT CAG GTA TGA TTT AA A TGG TCA GT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSMART-ala12 was a gift from Michael Lynch (Addgene plasmid # 87173 ; http://n2t.net/addgene:87173 ; RRID:Addgene_87173) -
For your References section:
Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli. Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD. Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30. 10.1016/j.ymben.2021.09.009 PubMed 34600151