pAAV-CBh-DIO-EGFP
(Plasmid #87168)

• Purpose: Expression of eGFP in the presence of Cre
• Depositing Lab: Jimok Kim
• Publication: Li et al Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053.

Backbone

• Vector backbone: pAAV-Ef1a-DIO-EGFP-WPRE-pA
• Backbone manufacturer: K. Deisseroth Lab (Addgene #37084)
• Backbone size w/o insert (bp): 6342
• Total vector size (bp): 5295
• Modifications to backbone: 1. The EF1α promoter between the restriction sites MluI and BamHI in the backbone plasmid was replaced by the CBh promoter, which was PCR-amplified from the plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230). 2. The WPRE-pA between EcoRI and BstEII sites in the backbone plasmid was replaced by the CW3SL sequence, which was amplified by PCR from the plasmid pAAV-CW3SL-EGFP (Addgene #61463).
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Species: Synthetic
• Insert Size (bp): 846
• Tag / Fusion Protein:
  • None

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry