pCASCADE-fabI-gltA1-gltA2-udhA-zwf
(Plasmid
#87148)
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PurposefabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCASCADE
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Backbone manufacturerNone - Constructed in House
- Backbone size w/o insert (bp) 2500
- Total vector size (bp) 2903
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namefabI-gltA1-gltA2-udhA-zwf guide array
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gRNA/shRNA sequence5 Guide Array : E . coli fabI, gltA promoter1, gltA promoter 2, udhA promoter, zwf promoter
- Promoter E . coli ugpB gene promoter, low phosphate induction
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CACCACGTCGTCCCTATCTG
- 3′ sequencing primer ATCTCCGCCCCGTTCGTAA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCASCADE-fabI-gltA1-gltA2-udhA-zwf was a gift from Michael Lynch (Addgene plasmid # 87148 ; http://n2t.net/addgene:87148 ; RRID:Addgene_87148)