pCDF-mcherry2
(Plasmid
#87145)
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PurposeConstitutive E. coli mcherry Expression, with DAS4 tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87145 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDF
- Backbone size w/o insert (bp) 2000
- Total vector size (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemcherry
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SpeciesSynthetic
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Insert Size (bp)1000
- Promoter Constitutive proD
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Tag
/ Fusion Protein
- C terminal DAS4 tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CAG TCC AGT TAC GCT GGA GTC
- 3′ sequencing primer GGT CAG GTA TGA TTT AA A TGG TCA GT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF-mcherry2 was a gift from Michael Lynch (Addgene plasmid # 87145 ; http://n2t.net/addgene:87145 ; RRID:Addgene_87145)
