pcDNA-RLuc8.6-535
(Plasmid #87125)

• Purpose: Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)
• Depositing Lab: Sanjiv Sam Gambhir
• Publication: Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2007 Jul 8.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5358
• Total vector size (bp): 6358
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Renilla Luciferase
• Alt name: Rluc
• Species: Sea pansy, Renilla reniformis
• Insert Size (bp): 933
• Mutation: RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry