pcDNA-RLuc8
(Plasmid #87121)

• Purpose: Mammalian expression vector expressing mutant vesrion of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.
• Depositing Lab: Sanjiv Sam Gambhir
• Publication: Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-400. Epub 2006 Jul 20.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5358
• Total vector size (bp): 6358
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Renilla Luciferase
• Alt name: Rluc
• Species: Sea pansy, Renilla reniformis
• Insert Size (bp): 933
• Mutation: A55T, C124A, S130A, K136R, A143M, M185V, M253L, and S287L
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry