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Stat92e pBluescript SK(+)
(Plasmid #8712)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8712 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stat92e
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3800
  • GenBank ID
    U46688
  • Entrez Gene
    Stat92E (a.k.a. Dmel_CG4257, CG4257, D-STAT, D-Stat, D-stat, D-stat/stat92E, DRODSRC, DSRC, DSTAT, DmSTAT, Dmel\CG4257, Dst, Dstat, Dstat 92E, Dstat92E, SD-stat, STAT, STAT 92E, STAT92, STAT92E, STAT92e, Sta92E, Stat, Stat1alpha-like, Stat92, Stat92e, d-STAT, dSTAT, dSTAT92E, dSTAT92E/marelle, dstat, l(3)06346, l(3)j6C8, mrL, mrl, mrl stat92E, pSTAT, pSTAT92E, stat, stat92, stat92E, stat92e, stat[92E])

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned into EcoRI and XhoI, but the insert can be removed with EcoRI and KpnI. Diagnostic Restriction Digest with HindIII and KpnI (3.0kb and 3.8kb).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat92e pBluescript SK(+) was a gift from Jim Darnell (Addgene plasmid # 8712 ; http://n2t.net/addgene:8712 ; RRID:Addgene_8712)

  • For your References section:

    Identification of a Stat gene that functions in Drosophila development. Yan R, Small S, Desplan C, Dearolf CR, Darnell JE Jr. Cell 1996 Feb 9;84(3):421-30. 10.1016/S0092-8674(00)81287-8 PubMed 8608596
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