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Addgene

Stat4 pRc/CMV
(Plasmid #8711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stat4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    U06923
  • Entrez Gene
    Stat4

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Diagnostic restriction digest with BamHI (3.1kb, 2.0kb, 1.9kb, and 0.9kb).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat4 pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8711 ; http://n2t.net/addgene:8711 ; RRID:Addgene_8711)

  • For your References section:

    Stat3 and Stat4: members of the family of signal transducers and activators of transcription. Zhong Z, Wen Z, Darnell JE Jr. Proc Natl Acad Sci U S A 1994 May 24;91(11):4806-10. 10.1073/pnas.91.11.4806 PubMed 7545930
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