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pET28b-pchG WT
(Plasmid #87107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 6418
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    20 hours growth after induction
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pchG WT
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    1050
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pET upstream
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-pchG WT was a gift from Audrey Lamb (Addgene plasmid # 87107 ; http://n2t.net/addgene:87107 ; RRID:Addgene_87107)

  • For your References section:

    Holo Structure and Steady State Kinetics of the Thiazolinyl Imine Reductases for Siderophore Biosynthesis. Meneely KM, Ronnebaum TA, Riley AP, Prisinzano TE, Lamb AL. Biochemistry. 2016 Sep 27;55(38):5423-33. doi: 10.1021/acs.biochem.6b00735. Epub 2016 Sep 15. 10.1021/acs.biochem.6b00735 PubMed 27601130
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