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Addgene

pET28b-irp3 WT
(Plasmid #87106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 6466
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    18 hours growth after induction
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    irp3 WT
  • Species
    Yersinia enterocolitica
  • Insert Size (bp)
    1098
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pET upstream
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Biochemistry 55 (2016) 5423-5433

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-irp3 WT was a gift from Audrey Lamb (Addgene plasmid # 87106 ; http://n2t.net/addgene:87106 ; RRID:Addgene_87106)

  • For your References section:

    Two structures of a thiazolinyl imine reductase from Yersinia enterocolitica provide insight into catalysis and binding to the nonribosomal peptide synthetase module of HMWP1. Meneely KM, Lamb AL. Biochemistry. 2012 Nov 6;51(44):9002-13. doi: 10.1021/bi3011016. Epub 2012 Oct 23. 10.1021/bi3011016 PubMed 23066849
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