pET29b-irp9 WT
(Plasmid #87105)

• Purpose: expresses Irp9 WT, the salicylate synthase from Yersinia enterocolitica
• Depositing Lab: Audrey Lamb
• Publication: Meneely et al Arch Biochem Biophys. 2013 Nov 1;539(1):70-80. doi: 10.1016/j.abb.2013.09.007. Epub 2013 Sep 19.

Backbone

• Vector backbone: pET29b
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 6672
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grown at 37 degrees until OD600 ~ 1.0. Temperature turned to 18 degrees for 20 hours without induction
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: irp9 WT
• Species: Yersinia enterocolitica
• Insert Size (bp): 1302
• Promoter: T7
• Tag / Fusion Protein:
  • his-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pET upstream
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

JACS 138 (2016) 9277-9293 PMC5029964