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Addgene

pET29b-irp9 WT
(Plasmid #87105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 6672
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grown at 37 degrees until OD600 ~ 1.0. Temperature turned to 18 degrees for 20 hours without induction
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    irp9 WT
  • Species
    Yersinia enterocolitica
  • Insert Size (bp)
    1302
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pET upstream
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JACS 138 (2016) 9277-9293 PMC5029964

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-irp9 WT was a gift from Audrey Lamb (Addgene plasmid # 87105 ; http://n2t.net/addgene:87105 ; RRID:Addgene_87105)

  • For your References section:

    Redesign of MST enzymes to target lyase activity instead promotes mutase and dehydratase activities. Meneely KM, Luo Q, Lamb AL. Arch Biochem Biophys. 2013 Nov 1;539(1):70-80. doi: 10.1016/j.abb.2013.09.007. Epub 2013 Sep 19. 10.1016/j.abb.2013.09.007 PubMed 24055536
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