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pET28b-pvdA WT
(Plasmid #87098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    3-4 hours growth after induction
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pvdA WT
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    1332
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pet upstream
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Biochemistry, 2007, 46, 11930-11937, PMC2597334
Biochemistry 2009, 48, 4371-4376, PMC2710847

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-pvdA WT was a gift from Audrey Lamb (Addgene plasmid # 87098 ; http://n2t.net/addgene:87098 ; RRID:Addgene_87098)

  • For your References section:

    Two structures of an N-hydroxylating flavoprotein monooxygenase: ornithine hydroxylase from Pseudomonas aeruginosa. Olucha J, Meneely KM, Chilton AS, Lamb AL. J Biol Chem. 2011 Sep 9;286(36):31789-98. doi: 10.1074/jbc.M111.265876. Epub 2011 Jul 13. 10.1074/jbc.M111.265876 PubMed 21757711
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