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Addgene

GLI2 his
(Plasmid #87097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1/His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 10258
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI2
  • Alt name
    GLI family zinc finger 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4758
  • GenBank ID
    NM_005270.4
  • Entrez Gene
    GLI2 (a.k.a. CJS, HPE9, PHS2, THP1, THP2)
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • T7 tag (N terminal on backbone)
    • Xpress tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GLI2 his was a gift from Martin Fernandez-Zapico (Addgene plasmid # 87097 ; http://n2t.net/addgene:87097 ; RRID:Addgene_87097)
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