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Purpose(Empty Backbone) Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6.2
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
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Tag
/ Fusion Protein
- Nanoluc luciferase (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Nanoluc luciferase (C terminal on backbone)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Slow-growing, requires 3 days growth at 30C
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6.2-ccdB-Nanoluc was a gift from Mikko Taipale (Addgene plasmid # 87075 ; http://n2t.net/addgene:87075 ; RRID:Addgene_87075)