6xHis-TEV-GEI-17(133-509)
(Plasmid #87056)

• Purpose: Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF
• Depositing Lab: Ronald Hay
• Publication: Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485.

Backbone

• Vector backbone: pHISTEV30a
• Backbone size w/o insert (bp): 5365
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GEI-17 isfoomr f (aa 133-509)
• Alt name: C. elegans SUMO E3 ligase (PIAS family)
• Species: C. elegans (nematode)
• Entrez Gene: gei-17 (a.k.a. CELE_W10D5.3)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis-TEV (N terminal on backbone)
  • GAGGGS linker (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has a GAGGGS linker that improves TEV cleavage efficiency.