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Addgene

6xHis-TEV-UBC-9
(Plasmid #87055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PpHISTEV30a
  • Backbone size w/o insert (bp) 5365
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UBC-9
  • Alt name
    C. elegans Ubc9
  • Species
    C. elegans (nematode)
  • Entrez Gene
    ubc-9 (a.k.a. CELE_F29B9.6)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHis-TEV-UBC-9 was a gift from Ronald Hay (Addgene plasmid # 87055 ; http://n2t.net/addgene:87055 ; RRID:Addgene_87055)

  • For your References section:

    Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans. Pelisch F, Sonneville R, Pourkarimi E, Agostinho A, Blow JJ, Gartner A, Hay RT. Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485. 10.1038/ncomms6485 PubMed 25475837
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