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Addgene

Stat2 pBluescript SK (-)
(Plasmid #8705)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8705 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SK(-)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stat2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • GenBank ID
    M97934
  • Entrez Gene
    STAT2 (a.k.a. IMD44, ISGF-3, P113, PTORCH3, STAT113)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Diagnostic Restriction Digest with HindIII (2.1kb and 3.5kb).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat2 pBluescript SK (-) was a gift from Jim Darnell (Addgene plasmid # 8705 ; http://n2t.net/addgene:8705 ; RRID:Addgene_8705)
  • For your References section:

    Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr. Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7836-9. 10.1073/pnas.89.16.7836 PubMed 1502203