Stat1 beta Flag pRc/CMV
(Plasmid #8704)

• Depositing Lab: Jim Darnell
• Publication: Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7836-9.

Backbone

• Vector backbone: pRc/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAT1 beta
• Alt name: STAT1b
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• GenBank ID: M97936
• Entrez Gene: STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
• Tag / Fusion Protein:
  • Flag Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb).