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Addgene

pBS_flGAD
(Plasmid #87031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 4721
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    flGAD
  • Alt name
    glutamate decarboxylase 2
  • Alt name
    GAD65
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1871
  • GenBank ID
    NM_000818.2
  • Entrez Gene
    GAD2 (a.k.a. GAD65)
  • Promoter Lac-Z

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not 1 (unknown if destroyed)
  • 3′ cloning site Kpn1 (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS_flGAD was a gift from Janice Blum (Addgene plasmid # 87031 ; http://n2t.net/addgene:87031 ; RRID:Addgene_87031)
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