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Addgene

Stat1 alpha Y701F Flag pRc/CMV
(Plasmid #8702)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT1 alpha Y701F
  • Alt name
    STAT1a
  • Alt name
    STAT1 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    Y701F
  • GenBank ID
    M97935
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb). Author's Map shows the wild-type version of this plasmid (without the Y701F mutation).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat1 alpha Y701F Flag pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8702 ; http://n2t.net/addgene:8702 ; RRID:Addgene_8702)

  • For your References section:

    Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Wen Z, Zhong Z, Darnell JE Jr. Cell 1995 Jul 28;82(2):241-50. 10.1016/0092-8674(95)90311-9 PubMed 7543024
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