jk67
(Plasmid
#87013)
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Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screen
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSinEGdsp
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Vector typeMammalian Expression, Synthetic Biology ; SIndbis virus genomic plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCLIP-Nrx1B-4xλN(i)
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SpeciesM. musculus (mouse)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemCherry
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
jk67 was a gift from Anthony Zador (Addgene plasmid # 87013 ; http://n2t.net/addgene:87013 ; RRID:Addgene_87013) -
For your References section:
Using high-throughput barcode sequencing to efficiently map connectomes. Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, Correa IR Jr, Bressan D, Zador AM. Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292. 10.1093/nar/gkx292 PubMed 28449067