IDP455
(Plasmid
#87001)
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Purposeplasmid for cell culture expression of SYNseq components
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebarcode landing site Gfp-4xboxB
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SpeciesM. musculus (mouse)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IDP455 was a gift from Anthony Zador (Addgene plasmid # 87001 ; http://n2t.net/addgene:87001 ; RRID:Addgene_87001) -
For your References section:
Using high-throughput barcode sequencing to efficiently map connectomes. Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, Correa IR Jr, Bressan D, Zador AM. Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292. 10.1093/nar/gkx292 PubMed 28449067