pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP
(Plasmid
#86987)
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PurposeFor cloning and expression of sgRNA together with expression of Cas9 and TagRFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescript
- Backbone size w/o insert (bp) 2770
- Total vector size (bp) 11709
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
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SpeciesStreptococcus pyogenes
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Insert Size (bp)4206
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer M13 forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP was a gift from Ralf Kuehn (Addgene plasmid # 86987 ; http://n2t.net/addgene:86987 ; RRID:Addgene_86987) -
For your References section:
Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. Yumlu S, Bashir S, Stumm J, Kuhn R. Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10. 10.1007/978-1-4939-9170-9_10 PubMed 30912045
Map uploaded by the depositor.
