pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA
(Plasmid #86986)

• Purpose: For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein
• Depositing Lab: Ralf Kuehn
• Publication: Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10.

Backbone

• Vector backbone: Bluescript
• Backbone size w/o insert (bp): 2770
• Total vector size (bp): 10146
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9-venus
• Species: Synthetic; Streptococcus pyogenes
• Insert Size (bp): 4938
• Promoter: CAG
• Tag / Fusion Protein:
  • Cas9-Venus fusion protein (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • Cloning of sgRNAs
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry