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U6-(BbsI)sgRNA_CAG-Venus-bpA
(Plasmid #86985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript(-)
  • Backbone size w/o insert (bp) 2770
  • Total vector size (bp) 5968
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus
  • Species
    Aquorea victoria
  • Insert Size (bp)
    741
  • Mutation
    Venus GFP variant
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-(BbsI)sgRNA_CAG-Venus-bpA was a gift from Ralf Kuehn (Addgene plasmid # 86985 ; http://n2t.net/addgene:86985 ; RRID:Addgene_86985)

  • For your References section:

    Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. Yumlu S, Bashir S, Stumm J, Kuhn R. Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10. 10.1007/978-1-4939-9170-9_10 PubMed 30912045
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