pD603 H77 E1E2
(Plasmid
#86983)
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PurposeMammalian expression vector encoding codon optimised Heptitis C virus H77 glycoproteins E1E2
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepD603
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Backbone manufacturerDNA 2.0
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHCV E1E2
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Alt nameAAB67036.1
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: The HCV E1E2 insert contains an N391S mutation compared to AAB67036.1. The encoded protein is based on the consensus H77 sequence (AF009606), as described in https://www.ncbi.nlm.nih.gov/pubmed/9228008.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD603 H77 E1E2 was a gift from Joe Grove (Addgene plasmid # 86983 ; http://n2t.net/addgene:86983 ; RRID:Addgene_86983)
