pK19pA-MN
(Plasmid #86973)

• Purpose: ChIC; genomic mapping of chromatin proteins
• Depositing Lab: Ulrich Laemmli
• Publication: Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

Backbone

• Vector backbone: pK19
• Backbone size w/o insert (bp): 2661
• Total vector size (bp): 3534
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
• Species: Staphylococcus aureus
• Insert Size (bp): 927
• GenBank ID: Genbank P00644 GenBank: CP018205.1

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expresses a pA-MN fusion protein in E. coli. The MN coding sequence was cloned into pK19SEVII (Giraud-Panis et al., 1995). The pA segment contains 2 IgG binding domains of staphylococcal protein A and was fused to MN with a linker sequence of DDDKEF.