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Addgene

LeGO-lnc-MONC
(Plasmid #86966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LEGO-lnc
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin ; Cerulean

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MONC isoform 6
  • Alt name
    MIR99AHG
  • Alt name
    LINC00478
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR99AHG (a.k.a. C21orf34, C21orf35, LINC00478, MONC)
  • Promoter SFFV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer SFFV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LeGO-lnc-MONC was a gift from Jan-Henning Klusmann (Addgene plasmid # 86966 ; http://n2t.net/addgene:86966 ; RRID:Addgene_86966)

  • For your References section:

    LincRNAs MONC and MIR100HG act as oncogenes in acute megakaryoblastic leukemia. Emmrich S, Streltsov A, Schmidt F, Thangapandi VR, Reinhardt D, Klusmann JH. Mol Cancer. 2014 Jul 15;13:171. doi: 10.1186/1476-4598-13-171. 10.1186/1476-4598-13-171 PubMed 25027842
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