Sperm whale Mb 64Y68F in pVP80K
(Plasmid
#86956)
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PurposeIncreases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVP80K
- Backbone size w/o insert (bp) 4069
- Total vector size (bp) 4504
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Modifications to backboneadded rbs
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlease contact the Olson lab for expression instructions
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMb 64Y68F
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Speciessperm whale
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Insert Size (bp)500
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Mutation64Y68F
- Promoter T5
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T5 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sperm whale Mb 64Y68F in pVP80K was a gift from John Olson (Addgene plasmid # 86956 ; http://n2t.net/addgene:86956 ; RRID:Addgene_86956)
