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Addgene

pmrROSA-DEST
(Plasmid #86953)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmrROSA-DEST
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    This plasmid also contains Chloramphenicol resistance, but the plasmid grows better in ampicillin only. 30C growth reduces the toxicity of the plasmid and increases the DNA yield 3-4 fold compared to 37C. Grows very slowly and the colonies are very small.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A modified version of this plasmid was used in Sakers, K., Liu, Y., Llaci, L. et al. Loss of Quaking RNA binding protein disrupts the expression of genes associated with astrocyte maturation in mouse brain. Nat Commun 12, 1537 (2021). https://doi.org/10.1038/s41467-021-21703-5 (PMID: 33750804). Please refer to that article for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmrROSA-DEST was a gift from Joseph Dougherty (Addgene plasmid # 86953 ; http://n2t.net/addgene:86953 ; RRID:Addgene_86953)
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