pmrROSA-DEST
(Plasmid #86953)

• Purpose: (Empty Backbone) Gateway destination vector for targeting the ROSA locus for conditional mouse knock-ins.
• Depositing Lab: Joseph Dougherty
• Publication: Mitra lab plasmids (unpublished)

Backbone

• Vector backbone: pmrROSA-DEST
• Vector type: Mammalian Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: This plasmid also contains Chloramphenicol resistance, but the plasmid grows better in ampicillin only. 30C growth reduces the toxicity of the plasmid and increases the DNA yield 3-4 fold compared to 37C. Grows very slowly and the colonies are very small.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A modified version of this plasmid was used in Sakers, K., Liu, Y., Llaci, L. et al. Loss of Quaking RNA binding protein disrupts the expression of genes associated with astrocyte maturation in mouse brain. Nat Commun 12, 1537 (2021). https://doi.org/10.1038/s41467-021-21703-5 (PMID: 33750804). Please refer to that article for additional information.