pPgCdH3
(Plasmid
#8695)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonena
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecid promoter, H3
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Alt nameH3
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Alt namecid
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SpeciesD. melanogaster (fly)
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Entrez GeneHis3:CG31613 (a.k.a. Dmel_CG31613, CG31613, Dmel\CG31613, His3)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ahmad lab #k91.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPgCdH3 was a gift from Kami Ahmad (Addgene plasmid # 8695 ; http://n2t.net/addgene:8695 ; RRID:Addgene_8695) -
For your References section:
Centromeres are specialized replication domains in heterochromatin. Ahmad K, Henikoff S. J Cell Biol 2001 Apr 2;153(1):101-10. 10.1083/jcb.153.1.101 PubMed 11285277
