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Addgene

5LTR-puro-3LTR
(Plasmid #86948)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PiggyBac transposon
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5LTR-puro-3LTR was a gift from Rob Mitra (Addgene plasmid # 86948 ; http://n2t.net/addgene:86948 ; RRID:Addgene_86948)

  • For your References section:

    An optimized, broadly applicable piggyBac transposon induction system. Qi Z, Wilkinson MN, Chen X, Sankararaman S, Mayhew D, Mitra RD. Nucleic Acids Res. 2017 Jan 12. pii: gkw1290. doi: 10.1093/nar/gkw1290. 10.1093/nar/gkw1290 PubMed 28082389
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