Stx3 triple mutant (F31A D33A E34A)
(Plasmid #86945)

• Purpose: Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)
• Depositing Lab: Thomas Weimbs
• Publication: Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. doi: 10.1371/journal.pone.0163671. eCollection 2016.

Backbone

• Vector backbone: pcDNA4-TO
• Modifications to backbone: introduced a second myc epitope tag to make it myc-myc-His6
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STX3 triple mutant (F31A D33A E34A)
• Alt name: Syntaxin 3 triple mutant (F31A D33A E34A)
• Alt name: STX3
• Alt name: Syntaxin 3
• Species: H. sapiens (human)
• Mutation: triple mutant (F31A D33A E34A)
• GenBank ID: NM_004177
• Entrez Gene: STX3 (a.k.a. DIAR12, MVID2, RDMVID, STX3A)
• Tag / Fusion Protein:
  • myc-His6 (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry