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Addgene

CMV-dH3-YFP
(Plasmid #8694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 9800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H3
  • Alt name
    Histone H3
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1174
  • Entrez Gene
    His3:CG31613 (a.k.a. Dmel_CG31613, CG31613, Dmel\CG31613, His3)
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site Bgl II (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ahmad lab #k245.3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-dH3-YFP was a gift from Kami Ahmad (Addgene plasmid # 8694 ; http://n2t.net/addgene:8694 ; RRID:Addgene_8694)

  • For your References section:

    From silencing to gene expression: real-time analysis in single cells. Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL. Cell 2004 Mar 5;116(5):683-98. 10.1016/S0092-8674(04)00171-0 PubMed 15006351
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