Stat1 alpha Flag pRc/CMV
(Plasmid #8691)

• Depositing Lab: Jim Darnell
• Publication: Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Backbone

• Vector backbone: pRc/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAT1 alpha
• Alt name: STAT1a
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• GenBank ID:
• Entrez Gene: STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb).