Skip to main content
Addgene

pTXB1-AbaSFI
(Plasmid #86900)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pTXB1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AbaSDFI
  • Species
    Acenatobacter baumani
  • Promoter T7
  • Tag / Fusion Protein
    • CBD (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXB1-AbaSFI was a gift from Matthias Bochtler (Addgene plasmid # 86900 ; http://n2t.net/addgene:86900 ; RRID:Addgene_86900)

  • For your References section:

    Crystal structure of the 5hmC specific endonuclease PvuRts1I. Kazrani AA, Kowalska M, Czapinska H, Bochtler M. Nucleic Acids Res. 2014 May;42(9):5929-36. doi: 10.1093/nar/gku186. Epub 2014 Mar 14. 10.1093/nar/gku186 PubMed 24634440
Related items:
Commonly requested with: