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pET15b-D57A-PvuRts1I
(Plasmid #86892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PvuRts1I
  • Mutation
    D57A
  • Entrez Gene
    pvuRts1I (a.k.a. ST585_p002, Rts1_063)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-D57A-PvuRts1I was a gift from Matthias Bochtler (Addgene plasmid # 86892 ; http://n2t.net/addgene:86892 ; RRID:Addgene_86892)

  • For your References section:

    Crystal structure of the 5hmC specific endonuclease PvuRts1I. Kazrani AA, Kowalska M, Czapinska H, Bochtler M. Nucleic Acids Res. 2014 May;42(9):5929-36. doi: 10.1093/nar/gku186. Epub 2014 Mar 14. 10.1093/nar/gku186 PubMed 24634440
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