-
PurposeN-terminal part of the split-BioID
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1
- Total vector size (bp) 6987
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePP1g - FLAG - BirA (AA2-140)
-
SpeciesH. sapiens (human), R. norvegicus (rat)
-
MutationBirA Amino Acids 2-140
-
Tag
/ Fusion Protein
- FLAG tag
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation Q61R was found in the Neomycin cassette during Addgene's quality control process. The depositing lab has not tested neomycin selection for this plasmid.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1 - PP1g - Flag - BirA* (AA2-140) was a gift from Mathieu Bollen (Addgene plasmid # 86886 ; http://n2t.net/addgene:86886 ; RRID:Addgene_86886) -
For your References section:
Split-BioID: a proximity-biotinylation assay for dimerization-dependent protein interactions. Munter S, Gornemann J, Derua R, Lesage B, Qian J, Heroes E, Waelkens E, Eynde AV, Beullens M, Bollen M. FEBS Lett. 2016 Dec 29. doi: 10.1002/1873-3468.12548. 10.1002/1873-3468.12548 PubMed 28032891
