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Addgene

pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1
(Plasmid #86885)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86885 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA* (AA141-321)- HA- NIPP1]
  • Species
    H. sapiens (human), B. taurus (bovine)
  • Insert Size (bp)
    6800
  • Mutation
    BirA Amino Acids 141-321
  • Tag / Fusion Protein
    • HA tag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1 was a gift from Mathieu Bollen (Addgene plasmid # 86885 ; http://n2t.net/addgene:86885 ; RRID:Addgene_86885)
  • For your References section:

    Split-BioID: a proximity-biotinylation assay for dimerization-dependent protein interactions. Munter S, Gornemann J, Derua R, Lesage B, Qian J, Heroes E, Waelkens E, Eynde AV, Beullens M, Bollen M. FEBS Lett. 2016 Dec 29. doi: 10.1002/1873-3468.12548. 10.1002/1873-3468.12548 PubMed 28032891