pET30a-YedK
(Plasmid #86884)

• Purpose: Bacterial expression of E. coli SRAP ortholog YedK
• Depositing Lab: Douglas Feldman
• Publication: Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055.

Backbone

• Vector backbone: pET30a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YedK
• Alt name: duf159
• Alt name: yedk
• Species: Eschericia coli
• Insert Size (bp): 700
• GenBank ID: YedK 946435
• Entrez Gene: yedK (a.k.a. b1931, ECK1930, JW1916, yedG)
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • S-Tag (N terminal on backbone)
  • HA tag (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry