4xM67 pTATA TK-Luc
(Plasmid #8688)

• Depositing Lab: Jim Darnell
• Publication: Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

Backbone

• Vector backbone: pTATA TK-Luc
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 4 repeated M67 sites
• Insert Size (bp): 98

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AccI (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: lucNrev

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To obtain p4xM67-tk-Luc, an oligonucleotide containing four copies of the sequence GGTTCCCGTAAATGCATCA (TTCCCGTAA is the STAT-binding site) was introduced in the AccI-BamHI sites of pTATA-tk-Luc upstream of the minimal promoter. Diagnostic digest is EcoRI (3.6kb, 1.7kb, 0.6kb).