pcDNA5/FRT/TO-FLAG-CHK1
(Plasmid #86875)

• Purpose: To express CHK1 with a FLAG tag at N-terminus.
• Depositing Lab: Yihong Ye
• Publication: Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.

Backbone

• Vector backbone: pcDNA 5/FR/TO
• Backbone size w/o insert (bp): 5070
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CHEK1
• Alt name: CHK1
• Species: H. sapiens (human)
• Insert Size (bp): 1431
• Mutation: none
• GenBank ID: NM_001274.5.
• Entrez Gene: CHEK1 (a.k.a. CHK1, OZEMA21)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry